□ □□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□ □□□ □□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□ □□□□□□□□□□□□□□□□□□□ □□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□ □□□□□ □□□□□□□□□□□□(Nature 1997, PNAS 2012)□□□□□□□□□ □□□□□□ □□□□□□□□□□□□□□ (Nature 2005)、□□□□□□□□□□□□□ □□□□□□□□□□□□□□□□ □□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□ (Sci Rep 2013, 2015, Science 2018)□□ □ □ □ □□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□ □□□□□ □□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□(Science 2020, Nat Commun 2023)□□ □□□ □ □ □□□□□□□□□□□□□□ □□□□□□ □□□□□□□□ □ □ □ □ □ □ □ □ □ □ (PNAS 2020a, 2020b, 2022, 2023)□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□ □ □□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□ikawa@biken.osaka-u.ac.jphmiya003@biken.osaka-u.ac.jpshimada-k@biken.osaka-u.ac.jpemoric@biken.osaka-u.ac.jpkamoshita@biken.osaka-u.ac.jp mashiko@biken.osaka-u.ac.jp 図 3．精子鞭毛の経時的変化を約 0.1 秒間観察したところ、Ppp3cc 欠損精子では、中片部 ( 矢印 )が屈曲できず不妊になる こ と が 分 か っ た(Science. 2015)。図 4．レンチウイルスベクターを 2 細胞期胚に感染させると胎児と胎盤の両方に、胚盤胞期胚に感染させると胎盤特異的に遺伝子導入 で き る (Nat Biotechnol. 2007; PNAS. 2011)。Masahito IKAWA Haruhiko MIYATA Keisuke SHIMADA Chihiro EMORI Ppp3cc未感染□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□□教 授 伊川 正人 准 教 授 宮田 治彦 助 教 嶋田 圭祐 助 教 江森 千紘 助 教 鴨下 真紀 特任助教 増子 大輔 06-6879-8375 Professor 06-6879-8375 Associate Professor 06-6879-8375 Assistant Professor 06-6879-8375 Assistant Professor 06-6879-8375 Assistant Professor Maki KAMOSHITA Daisuke MASHIKO 06-6879-8375 Assistant Professor Fax 06-6879-8376+/-Ppp3cc-/-2 細胞胚盤胞教 授 伊川 正人 1. Kiyozumi D. et al., A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility. Nat Commun 14:2354 (2023) 2. Morohoshi A. et al., Testis-enriched ferlin, FER1L5, is required for Ca2+-activated acrosome reaction and male fertility. Sci Adv 9:eade7607 (2023) 3. Shimada K. et al., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. PNAS 120:e2221762120 (2023) 4. Lu Y. et al., 1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction. PNAS 120:e2207263120 (2023) 5. Tang S. et al., Human sperm TMEM95 binds eggs and facilitates membrane fusion. PNAS 119:e2207805119 (2022) 6. Mori M. et al., RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization. J Cell Biol 220:e202012001 (2021). 7. Fujihara Y. et al., The conserved fertility factor SPACA4/Bouncer has divergent modes of action in vertebrate fertilization. PNAS 118:e2108777118 (2021). 8. Miyata H. et al., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. PNAS 118:e2106673118 (2021). 9. Shimada K. et al., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. PNAS 118:e2018355118 (2021).10.Liu C. et al., Bi-allelic DNAH8 variants lead to multiple morphological abnormalities of the sperm flagella and primary male infertility. Am J Hum Genet 107:330 (2020)11.Kiyozumi D. et al., NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility. Science 368:1132 (2020).12.Noda T. et al., Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice. PNAS 117:11493 (2020).13.Fujihara Y. et al., Spermatozoa lacking fertilization influencing membrane protein (FIMP) fail to fuse with oocytes in mice. PNAS 117:9393 (2020).14.Miyata H. et al., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350:442-5 (2015)図 1．GFP を全身で発現し、紫外線を当てると緑色蛍光を発するグリーンマウス (FEBS Lett. 1997)図 2．頭 部 に GFP、尾 部 にRFP を 局 在 さ せ た ト ラ ン スジェニックマウスの精子(Exp Anim. 2010)□□□□□□□□□□ Experimental Genome Research
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